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Regeneration in starved planarians depends on TRiC/CCT subunits modulating the unfolded protein response
Author(s) -
GutiérrezGutiérrez Óscar,
Felix Daniel A,
Salvetti Alessandra,
Amro Elias M,
Thems Anne,
Pietsch Stefan,
Koeberle Andreas,
Rudolph K Lenhard,
GonzálezEstévez Cristina
Publication year - 2021
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202152905
Subject(s) - planarian , microbiology and biotechnology , regeneration (biology) , biology , endoplasmic reticulum , unfolded protein response , blastema
Planarians are able to stand long periods of starvation by maintaining adult stem cell pools and regenerative capacity. The molecular pathways that are needed for the maintenance of regeneration during starvation are not known. Here, we show that down‐regulation of chaperonin TRiC/CCT subunits abrogates the regeneration capacity of planarians during starvation, but TRiC/CCT subunits are dispensable for regeneration in fed planarians. Under starvation, they are required to maintain mitotic fidelity and for blastema formation. We show that TRiC subunits modulate the unfolded protein response (UPR) and are required to maintain ATP levels in starved planarians. Regenerative defects in starved CCT‐depleted planarians can be rescued by either chemical induction of mild endoplasmic reticulum stress, which leads to induction of the UPR, or by the supplementation of fatty acids. Together, these results indicate that CCT‐dependent UPR induction promotes regeneration of planarians under food restriction.