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FBXO2/SCF ubiquitin ligase complex directs xenophagy through recognizing bacterial surface glycan
Author(s) -
Yamada Akihiro,
Hikichi Miyako,
Nozawa Takashi,
Nakagawa Ichiro
Publication year - 2021
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202152584
Subject(s) - glycan , ubiquitin ligase , ubiquitin , dna ligase , microbiology and biotechnology , biology , ubiquitin protein ligases , computational biology , genetics , chemistry , gene , glycoprotein
Xenophagy, also known as antibacterial selective autophagy, degrades invading bacterial pathogens such as group A Streptococcus (GAS) to defend cells. Although invading bacteria are known to be marked with ubiquitin and selectively targeted by xenophagy, how ubiquitin ligases recognize invading bacteria is poorly understood. Here, we show that FBXO2, a glycoprotein‐specific receptor for substrate in the SKP1/CUL1/F‐box protein (SCF) ubiquitin ligase complex, mediates recognition of GlcNAc side chains of the GAS surface carbohydrate structure and promotes ubiquitin‐mediated xenophagy against GAS. FBXO2 targets cytosolic GAS through its sugar‐binding motif and GlcNAc expression on the GAS surface. FBXO2 knockout resulted in decreased ubiquitin accumulation on intracellular GAS and xenophagic degradation of bacteria. Furthermore, SCF components such as SKP1, CUL1, and ROC1 are required for ubiquitin‐mediated xenophagy against GAS. Thus, SCF FBXO2 recognizes GlcNAc residues of GAS surface carbohydrates and functions in ubiquitination during xenophagy.