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MPK3/6‐induced degradation of ARR1/10/12 promotes salt tolerance in Arabidopsis
Author(s) -
Yan Zhenwei,
Wang Junxia,
Wang Fengxia,
Xie Chuantian,
Lv Bingsheng,
Yu Zipeng,
Dai Shaojun,
Liu Xia,
Xia Guangmin,
Tian Huiyu,
Li Cuiling,
Ding Zhaojun
Publication year - 2021
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202152457
Subject(s) - arabidopsis , cytokinin , arabidopsis thaliana , ectopic expression , microbiology and biotechnology , signal transduction , biology , mutant , regulator , chemistry , biochemistry , auxin , gene
Cytokinins are phytohormones that regulate plant development, growth, and responses to stress. In particular, cytokinin has been reported to negatively regulate plant adaptation to high salinity; however, the molecular mechanisms that counteract cytokinin signaling and enable salt tolerance are not fully understood. Here, we provide evidence that salt stress induces the degradation of the cytokinin signaling components Arabidopsis ( Arabidopisis thaliana ) response regulator 1 (ARR1), ARR10 and ARR12. Furthermore, the stress‐activated mitogen‐activated protein kinase 3 (MPK3) and MPK6 interact with and phosphorylate ARR1/10/12 to promote their degradation in response to salt stress. As expected, salt tolerance is decreased in the mpk3 / 6 double mutant, but enhanced upon ectopic MPK3/MPK6 activation in an MKK5 DD line. Importantly, salt hypersensitivity phenotypes of the mpk3 / 6 line were significantly alleviated by mutation of ARR1/12. The above results indicate that MPK3/6 enhance salt tolerance in part via their negative regulation of ARR1/10/12 protein stability. Thus, our work reveals a new molecular mechanism underlying salt‐induced stress adaptation and the inhibition of plant growth, via enhanced degradation of cytokinin signaling components.