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Hsp90‐mediated regulation of DYRK3 couples stress granule disassembly and growth via mTORC1 signaling
Author(s) -
Mediani Laura,
Antoniani Francesco,
Galli Veronica,
Vinet Jonathan,
Carrà Arianna Dorotea,
Bigi Ilaria,
Tripathy Vadreenath,
Tiago Tatiana,
Cimino Marco,
Leo Giuseppina,
Amen Triana,
Kaganovich Daniel,
Cereda Cristina,
Pansarasa Orietta,
Mandrioli Jessica,
Tripathi Priyanka,
Troost Dirk,
Aronica Eleonora,
Buchner Johannes,
Goswami Anand,
Sterneckert Jared,
Alberti Simon,
Carra Serena
Publication year - 2021
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202051740
Subject(s) - mtorc1 , stress granule , microbiology and biotechnology , hsp90 , signal transduction , biology , heat shock protein , genetics , pi3k/akt/mtor pathway , gene , messenger rna , translation (biology)
Stress granules (SGs) are dynamic condensates associated with protein misfolding diseases. They sequester stalled mRNAs and signaling factors, such as the mTORC1 subunit raptor, suggesting that SGs coordinate cell growth during and after stress. However, the molecular mechanisms linking SG dynamics and signaling remain undefined. We report that the chaperone Hsp90 is required for SG dissolution. Hsp90 binds and stabilizes the dual‐specificity tyrosine‐phosphorylation‐regulated kinase 3 (DYRK3) in the cytosol. Upon Hsp90 inhibition, DYRK3 dissociates from Hsp90 and becomes inactive. Inactive DYRK3 is subjected to two different fates: it either partitions into SGs, where it is protected from irreversible aggregation, or it is degraded. In the presence of Hsp90, DYRK3 is active and promotes SG disassembly, restoring mTORC1 signaling and translation. Thus, Hsp90 links stress adaptation and cell growth by regulating the activity of a key kinase involved in condensate disassembly and translation restoration.