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EDC3 phosphorylation regulates growth and invasion through controlling P‐body formation and dynamics
Author(s) -
Bearss Jeremiah J,
Padi Sathish KR,
Singh Neha,
CardoVila Marina,
Song Jin H,
Mouneimne Ghassan,
Fernandes Nikita,
Li Yang,
Harter Matthew R,
Gard Jaime MC,
Cress Anne E,
Peti Wolfgang,
Nelson Andrew DL,
Buchan J Ross,
Kraft Andrew S,
Okumura Koichi
Publication year - 2021
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202050835
Subject(s) - phosphorylation , microbiology and biotechnology , biology , messenger rna , kinase , translation (biology) , serine , gene , biochemistry
Regulation of mRNA stability and translation plays a critical role in determining protein abundance within cells. Processing bodies (P‐bodies) are critical regulators of these processes. Here, we report that the Pim1 and 3 protein kinases bind to the P‐body protein enhancer of mRNA decapping 3 (EDC3) and phosphorylate EDC3 on serine (S)161, thereby modifying P‐body assembly. EDC3 phosphorylation is highly elevated in many tumor types, is reduced upon treatment of cells with kinase inhibitors, and blocks the localization of EDC3 to P‐bodies. Prostate cancer cells harboring an EDC3 S161A mutation show markedly decreased growth, migration, and invasion in tissue culture and in xenograft models. Consistent with these phenotypic changes, the expression of integrin β1 and α6 mRNA and protein is reduced in these mutated cells. These results demonstrate that EDC3 phosphorylation regulates multiple cancer‐relevant functions and suggest that modulation of P‐body activity may represent a new paradigm for cancer treatment.