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Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish
Author(s) -
GarcíaPoyatos Carolina,
Cogliati Sara,
Calvo Enrique,
HernansanzAgustín Pablo,
Lagarrigue Sylviane,
Magni Ricardo,
Botos Marius,
Langa Xavier,
Amati Francesca,
Vázquez Jesús,
Mercader Nadia,
Enríquez José Antonio
Publication year - 2020
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202050287
Subject(s) - library science , humanities , art , computer science
The oxidative phosphorylation ( OXPHOS ) system is a dynamic system in which the respiratory complexes coexist with super‐assembled quaternary structures called supercomplexes ( SC s). The physiological role of SC s is still disputed. Here, we used zebrafish to study the relevance of respiratory SC s. We combined immunodetection analysis and deep data‐independent proteomics to characterize these structures and found similar SC s to those described in mice, as well as novel SC s including III 2 + IV 2 , I + IV , and I + III 2 + IV 2 . To study the physiological role of SC s, we generated two null allele zebrafish lines for supercomplex assembly factor 1 ( scaf1 ). scaf1 −/− fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV . scaf1 −/− fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.