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Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA
Author(s) -
Rennie Martin L,
Lemonidis Kimon,
Arkinson Connor,
Chaugule Viduth K,
Clarke Mairi,
Streetley James,
Spagnolo Laura,
Walden Helen
Publication year - 2020
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202050133
Subject(s) - ubiquitin , fancd2 , dna repair , dna , microbiology and biotechnology , dna damage , deubiquitinating enzyme , biology , dna replication , biochemistry , chemistry , fanconi anemia , gene
The Fanconi anaemia (FA) pathway is a dedicated pathway for the repair of DNA interstrand crosslinks and is additionally activated in response to other forms of replication stress. A key step in the FA pathway is the monoubiquitination of each of the two subunits (FANCI and FANCD2) of the ID2 complex on specific lysine residues. However, the molecular function of these modifications has been unknown for nearly two decades. Here, we find that ubiquitination of FANCD2 acts to increase ID2's affinity for double‐stranded DNA via promoting a large‐scale conformational change in the complex. The resulting complex encircles DNA, by forming a secondary “Arm” ID2 interface. Ubiquitination of FANCI, on the other hand, largely protects the ubiquitin on FANCD2 from USP1‐UAF1 deubiquitination, with key hydrophobic residues of FANCI's ubiquitin being important for this protection. In effect, both of these post‐translational modifications function to stabilize a conformation in which the ID2 complex encircles DNA.