Premium
The Arabidopsis lnc RNA ASCO modulates the transcriptome through interaction with splicing factors
Author(s) -
Rigo Richard,
Bazin Jérémie,
RomeroBarrios Natali,
Moison Michaël,
Lucero Leandro,
Christ Aurélie,
Benhamed Moussa,
Blein Thomas,
Huguet Stéphanie,
Charon Céline,
Crespi Martin,
Ariel Federico
Publication year - 2020
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201948977
Subject(s) - spliceosome , transcriptome , biology , rna splicing , flagellin , rna binding protein , rna , alternative splicing , genetics , gene , microbiology and biotechnology , computational biology , gene expression , messenger rna
Alternative splicing ( AS ) is a major source of transcriptome diversity. Long noncoding RNA s (lnc RNA s) have emerged as regulators of AS through different molecular mechanisms. In Arabidopsis thaliana , the AS regulators NSR s interact with the ALTERNATIVE SPLICING COMPETITOR ( ASCO ) lnc RNA . Here, we analyze the effect of the knock‐down and overexpression of ASCO at the genome‐wide level and find a large number of deregulated and differentially spliced genes related to flagellin responses and biotic stress. In agreement, ASCO ‐silenced plants are more sensitive to flagellin. However, only a minor subset of deregulated genes overlaps with the AS defects of the nsra/b double mutant, suggesting an alternative way of action for ASCO . Using biotin‐labeled oligonucleotides for RNA ‐mediated ribonucleoprotein purification, we show that ASCO binds to the highly conserved spliceosome component PRP 8a. ASCO overaccumulation impairs the recognition of specific flagellin‐related transcripts by PRP 8a. We further show that ASCO also binds to another spliceosome component, SmD1b, indicating that it interacts with multiple splicing factors. Hence, lnc RNA s may integrate a dynamic network including spliceosome core proteins, to modulate transcriptome reprogramming in eukaryotes.