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PP2A phosphatase is required for dendrite pruning via actin regulation in Drosophila
Author(s) -
Wolterhoff Neele,
Gigengack Ulrike,
Rumpf Sebastian
Publication year - 2020
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201948870
Subject(s) - cofilin , biology , microbiology and biotechnology , dendrite (mathematics) , ecdysone , actin , metamorphosis , protein phosphatase 2 , actin cytoskeleton , pruning , dendritic spine , phosphatase , cytoskeleton , neuroscience , genetics , botany , phosphorylation , gene , geometry , mathematics , hippocampal formation , larva , cell
Large‐scale pruning, the developmentally regulated degeneration of axons or dendrites, is an important specificity mechanism during neuronal circuit formation. The peripheral sensory class IV dendritic arborization (c4da) neurons of Drosophila larvae specifically prune their dendrites at the onset of metamorphosis in an ecdysone‐dependent manner. Dendrite pruning requires local cytoskeleton remodeling, and the actin‐severing enzyme Mical is an important ecdysone target. In a screen for pruning factors, we identified the protein phosphatase 2 A (PP2A). PP 2A interacts genetically with the actin‐severing enzymes Mical and cofilin as well as other actin regulators during pruning. Moreover, Drosophila cofilin undergoes a change in localization at the onset of metamorphosis indicative of a change in actin dynamics. This change is abolished both upon loss of Mical and PP 2A. We conclude that PP 2A regulates actin dynamics during dendrite pruning.