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Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter‐organelle translocation
Author(s) -
Yamashiro Haruna,
Negishi Mayu,
Kinoshita Tatsuki,
Ishizu Hirotsugu,
Ohtani Hitoshi,
Siomi Mikiko C
Publication year - 2019
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201948769
Subject(s) - piwi interacting rna , gene silencing , organelle , rasirna , biology , transposable element , mitochondrion , chromosomal translocation , microbiology and biotechnology , genetics , gene , genome
Piwi and pi RNA form the pi RNA ‐induced silencing complex (pi RISC ) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–pi RISC precursor (pre‐pi RISC ) to mitochondria, where Zucchini‐dependent pi RISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre‐pi RISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre‐pi RISC from Yb bodies and the translocation to mitochondria. Piwi–pre‐pi RISC resist leaving Yb bodies until Armi binds Piwi–pre‐pi RISC through the pi RNA precursors. The lack of the Armi N‐terminus also blocks the Piwi–pre‐pi RISC exit from Yb bodies. Thus, Armi determines Piwi–pi RISC processing, in a multilayered manner, from precursor formation and quality control to inter‐organelle translocation for maturation.