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Golgi‐associated microtubules are fast cargo tracks and required for persistent cell migration
Author(s) -
Hao Huiwen,
Niu Jiahao,
Xue Boxin,
Su Qian Peter,
Liu Menghan,
Yang Junsheng,
Qin Jinshan,
Zhao Shujuan,
Wu Congying,
Sun Yujie
Publication year - 2020
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201948385
Subject(s) - beijing , biography , china , state (computer science) , library science , chinese academy of sciences , molecular cell biology , political science , biology , computer science , law , microbiology and biotechnology , algorithm
Microtubules derived from the Golgi (Golgi MT s) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT ‐dependent vesicular trafficking. Here, using super‐resolution stochastic optical reconstruction microscopy ( STORM ), we discovered that post‐Golgi cargos are more enriched on Golgi MT s and also surprisingly move much faster than on non‐Golgi MT s. We found that, compared to non‐Golgi MT s, Golgi MT s are morphologically more polarized toward the cell leading edge with significantly fewer inter‐ MT intersections. In addition, Golgi MT s are more stable and contain fewer lattice repair sites than non‐Golgi MT s. Our STORM /live‐cell imaging demonstrates that cargos frequently pause at the sites of both MT intersections and MT defects. Furthermore, by optogenetic maneuvering of cell direction, we demonstrate that Golgi MT s are essential for persistent cell migration but not for cells to change direction. Together, our study unveils the role of Golgi MT s in serving as a group of “fast tracks” for anterograde trafficking of post‐Golgi cargos.