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R3hdml regulates satellite cell proliferation and differentiation
Author(s) -
Sakamoto Kenichi,
Furuichi Yasuro,
Yamamoto Masashi,
Takahashi Megumi,
Akimoto Yoshihiro,
Ishikawa Takahiro,
Shimizu Takahiko,
Fujimoto Masanori,
TakadaWatanabe Aki,
Hayashi Aiko,
Mita Yoshitaka,
Manabe Yasuko,
Fujii Nobuharu L,
Ishibashi Ryoichi,
Maezawa Yoshiro,
Betsholtz Christer,
Yokote Koutaro,
Takemoto Minoru
Publication year - 2019
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201947957
Subject(s) - metropolitan area , medicine , promotion (chess) , graduate students , biomedical sciences , gerontology , family medicine , library science , medical education , political science , pathology , politics , computer science , law
In this study, we identified a previously uncharacterized skeletal satellite cell‐secreted protein, R3h domain containing‐like (R3hdml). Expression of R3hdml increases during skeletal muscle development and differentiation in mice. Body weight and skeletal muscle mass of R3hdml knockout (KO) mice are lower compared to control mice. Expression levels of cell cycle‐related markers, phosphorylation of Akt, and expression of insulin‐like growth factor within the skeletal muscle are reduced in R3hdml KO mice compared to control mice. Expression of R3hdml increases during muscle regeneration in response to cardiotoxin (CTX)‐induced muscle injury. Recovery of handgrip strength after CTX injection was significantly impaired in R3hdml KO mice, which is rescued by R3hdml. Our results indicate that R3hdml is required for skeletal muscle development, regeneration, and, in particular, satellite cell proliferation and differentiation.