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Radial somatic F‐actin organization affects growth cone dynamics during early neuronal development
Author(s) -
Meka Durga Praveen,
Scharrenberg Robin,
Zhao Bing,
Kobler Oliver,
König Theresa,
Schaefer Irina,
Schwanke Birgit,
Klykov Sergei,
Richter Melanie,
Eggert Dennis,
Windhorst Sabine,
Dotti Carlos G,
Kreutz Michael R,
Mikhaylova Marina,
Calderon de Anda Froylan
Publication year - 2019
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201947743
Subject(s) - growth cone , somatic cell , actin , microbiology and biotechnology , biology , dynamics (music) , neuroscience , genetics , gene , psychology , axon , pedagogy
The centrosome is thought to be the major neuronal microtubule‐organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro , recent work showed that isolated centrosomes could serve as an actin‐organizing center, raising the possibility that neuronal development may, in addition, require a centrosome‐based actin radial organization. Here, we report, using super‐resolution microscopy and live‐cell imaging of cultured rodent neurons, F‐actin organization around the centrosome with dynamic F‐actin aster‐like structures with F‐actin fibers extending and retracting actively. Photoactivation/photoconversion experiments and molecular manipulations of F‐actin stability reveal a robust flux of somatic F‐actin toward the cell periphery. Finally, we show that somatic F‐actin intermingles with centrosomal PCM‐1 (pericentriolar material 1 protein) satellites. Knockdown of PCM‐1 and disruption of centrosomal activity not only affect F‐actin dynamics near the centrosome but also in distal growth cones. Collectively, the data show a radial F‐actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers, hence sustaining initial neuronal development.