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An LTR retrotransposon‐derived lnc RNA interacts with RNF 169 to promote homologous recombination
Author(s) -
Deng Bing,
Xu Wenli,
Wang Zelin,
Liu Chang,
Lin Penghui,
Li Bin,
Huang Qiaojuan,
Yang Jianhua,
Zhou Hui,
Qu Lianghu
Publication year - 2019
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201847650
Subject(s) - homologous recombination , recombination , retrotransposon , microbiology and biotechnology , physics , biology , genetics , gene , transposable element , genome
LTR retrotransposons are abundant repetitive elements in the human genome, but their functions remain poorly understood. Here, we report the function and regulatory mechanism of an ERV ‐9 LTR retrotransposon‐derived lnc RNA called p53‐regulated lnc RNA for homologous recombination ( HR ) repair 1 ( PRLH 1) in human cells. PRLH 1 is highly expressed in p53‐mutated hepatocellular carcinoma ( HCC ) samples and promotes cell proliferation in p53‐mutated HCC cells, and its transcription is promoted by NF ‐Y and suppressed by p53. Mechanistically, PRLH 1 specifically binds to an uncharacterized domain of RNF 169 through two GCUUCA boxes in its 5′ terminal region to form a DNA repair complex that supplants 53 BP 1 at double‐strand break ( DSB ) sites and then promotes the initiation of HR repair. Notably, PRLH 1 is essential for the stabilization of RNF 169, acting as an RNA platform to recruit and assemble HR protein factors. This study characterizes PRLH 1 as a novel HR ‐promoting factor and provides new insights into the function and mechanism of LTR retrotransposon‐derived lnc RNA s.