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Zebrafish facial lymphatics develop through sequential addition of venous and non‐venous progenitors
Author(s) -
Eng Tiffany CY,
Chen Wenxuan,
Okuda Kazuhide S,
Misa June P,
Padberg Yvonne,
Crosier Kathryn E,
Crosier Philip S,
Hall Christopher J,
SchulteMerker Stefan,
Hogan Benjamin M,
Astin Jonathan W
Publication year - 2019
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201847079
Subject(s) - library science , medical school , excellence , medicine , medical education , political science , computer science , law
Lymphatic vessels are known to be derived from veins; however, recent lineage‐tracing experiments propose that specific lymphatic networks may originate from both venous and non‐venous sources. Despite this, direct evidence of a non‐venous lymphatic progenitor is missing. Here, we show that the zebrafish facial lymphatic network is derived from three distinct progenitor populations that add sequentially to the developing facial lymphatic through a relay‐like mechanism. We show that while two facial lymphatic progenitor populations are venous in origin, the third population, termed the ventral aorta lymphangioblast ( VA ‐L), does not sprout from a vessel; instead, it arises from a migratory angioblast cell near the ventral aorta that initially lacks both venous and lymphatic markers, and contributes to the facial lymphatics and the hypobranchial artery. We propose that sequential addition of venous and non‐venous progenitors allows the facial lymphatics to form in an area that is relatively devoid of veins. Overall, this study provides conclusive, live imaging‐based evidence of a non‐venous lymphatic progenitor and demonstrates that the origin and development of lymphatic vessels is context‐dependent.