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A variable undecad repeat domain in cavin1 regulates caveola formation and stability
Author(s) -
Tillu Vikas A,
Lim YeWheen,
Kovtun Oleksiy,
Mureev Sergey,
Ferguson Charles,
Bastiani Michele,
McMahon KerrieAnn,
Lo Harriet P,
Hall Thomas E,
Alexandrov Kirill,
Collins Brett M,
Parton Robert G
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201845775
Subject(s) - library science , computer science
Caveolae are plasma membrane invaginations involved in transport, signalling and mechanical membrane sensing in metazoans. Their formation depends upon multiple interactions between membrane‐embedded caveolins, lipids and cytosolic cavin proteins. Of the four cavin family members, only cavin1 is strictly required for caveola formation. Here, we demonstrate that an eleven residue (undecad) repeat sequence ( UC 1) exclusive to cavin1 is essential for caveolar localization and promotes membrane remodelling through binding to phosphatidylserine. In the notochord of mechanically stimulated zebrafish embryos, the UC 1 domain is required for caveolar stability and resistance to membrane stress. The number of undecad repeats in the cavin1 UC 1 domain varies throughout evolution, and we find that an increased number also correlates with increased caveolar stability. Lastly, we show that the cavin1 UC 1 domain induces dramatic remodelling of the plasma membrane when grafted into cavin2 suggesting an important role in membrane sculpting. Overall, our work defines a novel conserved cavin1 modular domain that controls caveolar assembly and stability.