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SNX 18 regulates ATG 9A trafficking from recycling endosomes by recruiting Dynamin‐2
Author(s) -
Søreng Kristiane,
Munson Michael J,
Lamb Christopher A,
Bjørndal Gunnveig T,
Pankiv Serhiy,
Carlsson Sven R,
Tooze Sharon A,
Simonsen Anne
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744837
Subject(s) - exhibition , library science , medicine , art history , art , computer science
Trafficking of mammalian ATG 9A between the Golgi apparatus, endosomes and peripheral ATG 9A compartments is important for autophagosome biogenesis. Here, we show that the membrane remodelling protein SNX 18, previously identified as a positive regulator of autophagy, regulates ATG 9A trafficking from recycling endosomes. ATG 9A is recruited to SNX 18‐induced tubules generated from recycling endosomes and accumulates in juxtanuclear recycling endosomes in cells lacking SNX 18. Binding of SNX 18 to Dynamin‐2 is important for ATG 9A trafficking from recycling endosomes and for formation of ATG 16L1‐ and WIPI 2‐positive autophagosome precursor membranes. We propose a model where upon autophagy induction, SNX 18 recruits Dynamin‐2 to induce budding of ATG 9A and ATG 16L1 containing membranes from recycling endosomes that traffic to sites of autophagosome formation.