Premium
Lipid droplet biogenesis is spatially coordinated at ER –vacuole contacts under nutritional stress
Author(s) -
Hariri Hanaa,
Rogers Sean,
Ugrankar Rupali,
Liu Yang Lydia,
Feathers J Ryan,
Henne W Mike
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744815
Subject(s) - vacuole , microbiology and biotechnology , biogenesis , chemistry , lipid droplet , biology , biochemistry , gene , cytoplasm
Eukaryotic cells store lipids in cytosolic organelles known as lipid droplets ( LD s). Lipid droplet bud from the endoplasmic reticulum ( ER ), and may be harvested by the vacuole for energy during prolonged periods of starvation. How cells spatially coordinate LD production is poorly understood. Here, we demonstrate that yeast ER–vacuole contact sites ( NVJ s) physically expand in response to metabolic stress, and serve as sites for LD production. NVJ tether Mdm1 demarcates sites of LD budding, and interacts with fatty acyl‐CoA synthases at the NVJ periphery. Artificially expanding the NVJ through over‐expressing Mdm1 is sufficient to drive NVJ ‐associated LD production, whereas ablating the NVJ induces defects in fatty acid‐to‐triglyceride production. Collectively, our data suggest a tight metabolic link between nutritional stress and LD biogenesis that is spatially coordinated at ER –vacuole contact sites.