Premium
PAWS 1 controls Wnt signalling through association with casein kinase 1α
Author(s) -
Bozatzi Polyxeni,
Dingwell Kevin S,
Wu Kevin ZL,
Cooper Fay,
Cummins Timothy D,
Hutchinson Luke D,
Vogt Janis,
Wood Nicola T,
Macartney Thomas J,
Varghese Joby,
Gourlay Robert,
Campbell David G,
Smith James C,
Sapkota Gopal P
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744807
Subject(s) - casein kinase 2 , phosphorylation , casein kinase 1 , research council , library science , unit (ring theory) , operations research , protein kinase a , chemistry , engineering , computer science , biochemistry , philosophy , psychology , mitogen activated protein kinase kinase , linguistics , mathematics education , government (linguistics)
The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS 1 (also known as FAM 83G) interacts with SMAD 1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS 1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication. Consistent with these observations in Xenopus , Wnt signalling is diminished in U2 OS osteosarcoma cells lacking PAWS 1, while BMP signalling is unaffected. We show that PAWS 1 interacts and co‐localises with the α isoform of casein kinase 1 ( CK 1), and that PAWS 1 mutations incapable of binding CK 1 fail both to activate Wnt signalling and to elicit axis duplication in Xenopus embryos.