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The β3‐integrin endothelial adhesome regulates microtubule‐dependent cell migration
Author(s) -
Atkinson Samuel J,
Gontarczyk Aleksander M,
Alghamdi Abdullah AA,
Ellison Tim S,
Johnson Robert T,
Fowler Wesley J,
Kirkup Benjamin M,
Silva Bernardo C,
Harry Bronwen E,
Schneider Jochen G,
Weilbaecher Katherine N,
Mogensen Mette M,
Bass Mark D,
Parsons Maddy,
Edwards Dylan R,
Robinson Stephen D
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744578
Subject(s) - biological sciences , library science , biology , computational biology , computer science
Integrin β3 is seen as a key anti‐angiogenic target for cancer treatment due to its expression on neovasculature, but the role it plays in the process is complex; whether it is pro‐ or anti‐angiogenic depends on the context in which it is expressed. To understand precisely β3's role in regulating integrin adhesion complexes in endothelial cells, we characterised, by mass spectrometry, the β3‐dependent adhesome. We show that depletion of β3‐integrin in this cell type leads to changes in microtubule behaviour that control cell migration. β3‐integrin regulates microtubule stability in endothelial cells through Rcc2/Anxa2‐driven control of active Rac1 localisation. Our findings reveal that angiogenic processes, both in vitro and in vivo , are more sensitive to microtubule targeting agents when β3‐integrin levels are reduced.