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Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo‐tomography
Author(s) -
Gold Vicki AM,
Chroscicki Piotr,
Bragoszewski Piotr,
Chacinska Agnieszka
Publication year - 2017
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744261
Subject(s) - mitochondrion , ribosome , cytosol , electron tomography , microbiology and biotechnology , cryo electron tomography , tomography , biophysics , chemistry , biology , electron microscope , biochemistry , physics , rna , optics , enzyme , gene , scanning transmission electron microscopy
We employed electron cryo‐tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation‐arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.