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BRACHYURY directs histone acetylation to target loci during mesoderm development
Author(s) -
Beisaw Arica,
Tsaytler Pavel,
Koch Frederic,
Schmitz Sandra U,
Melissari MariaTheodora,
Senft Anna D,
Wittler Lars,
Pennimpede Tracie,
Macura Karol,
Herrmann Bernhard G,
Grote Phillip
Publication year - 2018
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744201
Subject(s) - brachyury , mesoderm , histone , acetylation , biology , genetics , computational biology , microbiology and biotechnology , gene , embryonic stem cell
T‐box transcription factors play essential roles in multiple aspects of vertebrate development. Here, we show that cooperative function of BRACHYURY (T) with histone‐modifying enzymes is essential for mouse embryogenesis. A single point mutation (T Y88A ) results in decreased histone 3 lysine 27 acetylation (H3K27ac) at T target sites, including the T locus, suggesting that T autoregulates the maintenance of its expression and functions by recruiting permissive chromatin modifications to putative enhancers during mesoderm specification. Our data indicate that T mediates H3K27ac recruitment through a physical interaction with p300. In addition, we determine that T plays a prominent role in the specification of hematopoietic and endothelial cell types. Hematopoietic and endothelial gene expression programs are disrupted in T Y88A mutant embryos, leading to a defect in the differentiation of hematopoietic progenitors. We show that this role of T is mediated, at least in part, through activation of a distal Lmo2 enhancer.