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Expansion of the ISWI chromatin remodeler family with new active complexes
Author(s) -
Oppikofer Mariano,
Bai Tianyi,
Gan Yutian,
Haley Benjamin,
Liu Peter,
Sandoval Wendy,
Ciferri Claudio,
Cochran Andrea G
Publication year - 2017
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201744011
Subject(s) - chromatin , genetics , biology , computational biology , computer science , microbiology and biotechnology , dna
ISWI chromatin remodelers mobilize nucleosomes to control DNA accessibility. Complexes isolated to date pair one of six regulatory subunits with one of two highly similar ATP ases. However, we find that each endogenously expressed ATP ase co‐purifies with every regulatory subunit, substantially increasing the diversity of ISWI complexes, and we additionally identify BAZ 2B as a novel, seventh regulatory subunit. Through reconstitution of catalytically active human ISWI complexes, we demonstrate that the new interactions described here are stable and direct. Finally, we profile the nucleosome remodeling functions of the now expanded family of ISWI chromatin remodelers. By revealing the combinatorial nature of ISWI complexes, we provide a basis for better understanding ISWI function in normal settings and disease.