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Nup358 binds to AGO proteins through its SUMO ‐interacting motifs and promotes the association of target mRNA with miRISC
Author(s) -
Sahoo Manas Ranjan,
Gaikwad Swati,
Khuperkar Deepak,
Ashok Maitreyi,
Helen Mary,
Yadav Santosh Kumar,
Singh Aditi,
Magre Indrasen,
Deshmukh Prachi,
Dhanvijay Supriya,
Sahoo Pabitra Kumar,
Ramtirtha Yogendra,
Madhusudhan Mallur Srivatsan,
Gayathri Pananghat,
Seshadri Vasudevan,
Joseph Jomon
Publication year - 2017
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201642386
Subject(s) - messenger rna , microbiology and biotechnology , rna binding protein , chemistry , biology , genetics , gene
Micro RNA (mi RNA )‐guided mRNA repression, mediated by the mi RNA ‐induced silencing complex (mi RISC ), is an important component of post‐transcriptional gene silencing. However, how mi RISC identifies the target mRNA in vivo is not well understood. Here, we show that the nucleoporin Nup358 plays an important role in this process. Nup358 localizes to the nuclear pore complex and to the cytoplasmic annulate lamellae ( AL ), and these structures dynamically associate with two mRNP granules: processing bodies (P bodies) and stress granules ( SG s). Nup358 depletion disrupts P bodies and concomitantly impairs the mi RNA pathway. Furthermore, Nup358 interacts with AGO and GW 182 proteins and promotes the association of target mRNA with mi RISC . A well‐characterized SUMO ‐interacting motif ( SIM ) in Nup358 is sufficient for Nup358 to directly bind to AGO proteins. Moreover, AGO and PIWI proteins interact with SIM s derived from other SUMO ‐binding proteins. Our study indicates that Nup358– AGO interaction is important for mi RNA ‐mediated gene silencing and identifies SIM as a new interacting motif for the AGO family of proteins. The findings also support a model wherein the coupling of mi RISC with the target mRNA could occur at AL , specialized domains within the ER , and at the nuclear envelope.