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A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two‐cell embryos
Author(s) -
Wang Jiaqiang,
Li Xin,
Wang Leyun,
Li Jingyu,
Zhao Yanhua,
Bou Gerelchimeg,
Li Yufei,
Jiao Guanyi,
Shen Xinghui,
Wei Renyue,
Liu Shichao,
Xie Bingteng,
Lei Lei,
Li Wei,
Zhou Qi,
Liu Zhonghua
Publication year - 2016
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201642051
Subject(s) - cleavage (geology) , rna , biology , embryo , microbiology and biotechnology , genetics , cell , non coding rna , gene , paleontology , fracture (geology)
Endogenous retroviruses ( ERV s) are transcriptionally active in cleavage stage embryos, yet their functions are unknown. ERV sequences are present in the majority of long intergenic noncoding RNA s (linc RNA s) in mouse and humans, playing key roles in many cellular processes and diseases. Here, we identify Linc GET as a nuclear linc RNA that is GLN ‐, MERVL ‐, and ERVK ‐associated and essential for mouse embryonic development beyond the two‐cell stage. Linc GET is expressed in late two‐ to four‐cell mouse embryos. Its depletion leads to developmental arrest at the late G2 phase of the two‐cell stage and to MAPK signaling pathway inhibition. Linc GET forms an RNA –protein complex with hn RNP U, FUBP 1, and ILF 2, promoting the cis‐regulatory activity of long terminal repeats ( LTR s) in GLN , MERVL , and ERVK ( GLKLTR s), and inhibiting RNA alternative splicing, partially by downregulating hn RNP U, FUBP 1, and ILF 2 protein levels. Hnrnpu or Ilf2 mRNA injection at the pronuclear stage also decreases the preimplantation developmental rate, and Fubp1 mRNA injection at the pronuclear stage causes a block at the two‐cell stage. Thus, as the first functional ERV ‐associated linc RNA , Linc GET provides clues for ERV functions in cleavage stage embryonic development.