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Centromere localization and function of Mis18 requires Yippee‐like domain‐mediated oligomerization
Author(s) -
Subramanian Lakxmi,
MedinaPritchard Bethan,
Barton Rachael,
Spiller Frances,
KulasegaranShylini Raghavendran,
Radaviciute Guoda,
Allshire Robin C,
Arockia Jeyaprakash A
Publication year - 2016
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201541520
Subject(s) - centromere , function (biology) , domain (mathematical analysis) , microbiology and biotechnology , biology , computational biology , computer science , genetics , gene , chromosome , mathematics , mathematical analysis
Mis18 is a key regulator responsible for the centromere localization of the CENP ‐A chaperone Scm3 in Schizosaccharomyces pombe and HJURP in humans, which establishes CENP ‐A chromatin that defines centromeres. The molecular and structural determinants of Mis18 centromere targeting remain elusive. Here, by combining structural, biochemical, and yeast genetic studies, we show that the oligomerization of S. pombe Mis18, mediated via its conserved N‐terminal Yippee‐like domain, is crucial for its centromere localization and function. The crystal structure of the N‐terminal Yippee‐like domain reveals a fold containing a cradle‐shaped pocket that is implicated in protein/nucleic acid binding, which we show is required for Mis18 function. While the N‐terminal Yippee‐like domain forms a homodimer in vitro and in vivo , full‐length Mis18, including the C‐terminal α‐helical domain, forms a homotetramer in vitro . We also show that the Yippee‐like domains of human Mis18α/Mis18β interact to form a heterodimer, implying a conserved structural theme for Mis18 regulation.