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The P af1 complex factors L eo1 and P af1 promote local histone turnover to modulate chromatin states in fission yeast
Author(s) -
Sadeghi Laia,
Prasad Punit,
Ekwall Karl,
Cohen Amikam,
Svensson J Peter
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201541214
Subject(s) - yeast , microbiology and biotechnology , chemistry , biology , biochemistry
The maintenance of open and repressed chromatin states is crucial for the regulation of gene expression. To study the genes involved in maintaining chromatin states, we generated a random mutant library in S chizosaccharomyces pombe and monitored the silencing of reporter genes inserted into the euchromatic region adjacent to the heterochromatic mating type locus. We show that L eo1– P af1 [a subcomplex of the RNA polymerase II ‐associated factor 1 complex ( P af1 C )] is required to prevent the spreading of heterochromatin into euchromatin by mapping the heterochromatin mark H 3 K 9me2 using high‐resolution genomewide C h IP ( C h IP –exo). Loss of L eo1– P af1 increases heterochromatin stability at several facultative heterochromatin loci in an RNA i‐independent manner. Instead, deletion of L eo1 decreases nucleosome turnover, leading to heterochromatin stabilization. Our data reveal that L eo1– P af1 promotes chromatin state fluctuations by enhancing histone turnover.