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Dead end1 is an essential partner of NANOS 2 for selective binding of target RNA s in male germ cell development
Author(s) -
Suzuki Atsushi,
Niimi Yuki,
Shinmyozu Kaori,
Zhou Zhi,
Kiso Makoto,
Saga Yumiko
Publication year - 2016
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201540828
Subject(s) - wheat germ , rna , chemistry , biology , microbiology and biotechnology , biochemistry , gene
RNA ‐binding proteins ( RBP s) play important roles for generating various cell types in many developmental processes, including eggs and sperms. Nanos is widely known as an evolutionarily conserved RNA ‐binding protein implicated in germ cell development. Mouse NANOS 2 interacts directly with the CCR 4‐ NOT ( CNOT ) deadenylase complex, resulting in the suppression of specific RNA s. However, the mechanisms involved in target specificity remain elusive. We show that another RBP , Dead end1 ( DND 1), directly interacts with NANOS 2 to load unique RNA s into the CNOT complex. This interaction is mediated by the zinc finger domain of NANOS 2, which is essential for its association with target RNA s. In addition, the conditional deletion of DND 1 causes the disruption of male germ cell differentiation similar to that observed in Nanos2 ‐ KO mice. Thus, DND 1 is an essential partner for NANOS 2 that leads to the degradation of specific RNA s. We also present the first evidence that the zinc finger domain of Nanos acts as a protein‐interacting domain for another RBP , providing a novel insight into Nanos‐mediated germ cell development.