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Ng BR is essential for endothelial cell glycosylation and vascular development
Author(s) -
Park Eon Joo,
Grabińska Kariona A,
Guan Ziqiang,
Sessa William C
Publication year - 2016
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201540789
Subject(s) - glycosylation , microbiology and biotechnology , chemistry , biochemistry , biology
Ng BR is a transmembrane protein identified as a Nogo‐B‐interacting protein and recently has been shown to be a subunit required for cis‐prenyltransferase (cis PT ase) activity. To investigate the integrated role of Ng BR in vascular development, we have characterized endothelial‐specific Ng BR knockout embryos. Here, we show that endothelial‐specific Ng BR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of Ng BR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR 2, VE ‐cadherin, and CD 31, and defective glycosylation can be rescued by treatment with the end product of cis PT ase activity, dolichol phosphate. Moreover, Ng BR functions in endothelial cells during embryogenesis are Nogo‐B independent. These data uniquely show the importance of Ng BR and protein glycosylation during vascular development.