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A BRCA 1‐interacting lnc RNA regulates homologous recombination
Author(s) -
Sharma Vivek,
Khurana Simran,
Kubben Nard,
Abdelmohsen Kotb,
Oberdoerffer Philipp,
Gorospe Myriam,
Misteli Tom
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201540437
Subject(s) - homologous recombination , homologous chromosome , rna , microbiology and biotechnology , biology , genetics , gene
Long non‐coding RNA s (lnc RNA s) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response ( DDR ). Using a microarray screen, we identify here a novel lnc RNA , DDSR 1 ( DNA damage‐sensitive RNA 1), which is induced upon DNA damage. DDSR 1 induction is triggered in an ATM ‐ NF ‐ κB pathway‐dependent manner by several DNA double‐strand break ( DSB ) agents. Loss of DDSR 1 impairs cell proliferation and DDR signaling and reduces DNA repair capacity by homologous recombination ( HR ). The HR defect in the absence of DDSR 1 is marked by aberrant accumulation of BRCA 1 and RAP 80 at DSB sites. In line with a role in regulating HR , DDSR 1 interacts with BRCA 1 and hn RNPUL 1, an RNA ‐binding protein involved in DNA end resection. Our results suggest a role for the lnc RNA DDSR 1 in modulating DNA repair by HR .