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Removal of H2A.Z by INO 80 promotes homologous recombination
Author(s) -
Alatwi Hanan E,
Downs Jessica A
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201540330
Subject(s) - chromatin , homologous recombination , microbiology and biotechnology , histone h2a , histone , biology , homologous chromosome , dna repair , chaperone (clinical) , rad51 , dna , genetics , gene , medicine , pathology
The mammalian INO 80 remodelling complex facilitates homologous recombination ( HR ), but the mechanism by which it does this is unclear. Budding yeast INO 80 can remove H2A.Z/H2B dimers from chromatin and replace them with H2A/H2B dimers. H2A.Z is actively incorporated at sites of damage in mammalian cells, raising the possibility that H2A.Z may need to be subsequently removed for resolution of repair. Here, we show that H2A.Z in human cells is indeed rapidly removed from chromatin flanking DNA damage by INO 80. We also report that the histone chaperone ANP 32E, which is implicated in removing H2 AZ from chromatin, similarly promotes HR and appears to work on the same pathway as INO 80 in these assays. Importantly, we demonstrate that the HR defect in cells depleted of INO 80 or ANP 32E can be rescued by H2A.Z co‐depletion, suggesting that H2A.Z removal from chromatin is the primary function of INO 80 and ANP 32E in promoting homologous recombination.