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The RNA surveillance complex P elo‐ H bs1 is required for transposon silencing in the D rosophila germline
Author(s) -
Yang Fu,
Zhao Rui,
Fang Xiaofeng,
Huang Huanwei,
Xuan Yang,
Ma Yanting,
Chen Hongyan,
Cai Tao,
Qi Yijun,
Xi Rongwen
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201540084
Subject(s) - piwi interacting rna , gene silencing , germline , transposable element , biology , argonaute , genetics , rasirna , drosophila melanogaster , rna interference , microbiology and biotechnology , mutant , rna silencing , rna , gene
Silencing of transposable elements ( TE s) in the metazoan germline is critical for genome integrity and is primarily dependent on Piwi proteins and associated RNA s, which exert their function through both transcriptional and posttranscriptional mechanisms. Here, we report that the evolutionarily conserved Pelo (Dom34)‐Hbs1 mRNA surveillance complex is required for transposon silencing in the D rosophila germline. In pelo mutant gonads, mRNA s and proteins of some selective TE s are up‐regulated. Pelo is not required for pi RNA biogenesis, and our studies suggest that Pelo may function at the translational level to silence TE s: This function requires interaction with Hbs1, and overexpression of RpS30a partially reverts TE ‐silencing defects in pelo mutants. Interestingly, TE silencing and spermatogenesis defects in pelo mutants can also effectively be rescued by expressing the mammalian ortholog of Pelo. We propose that the Pelo‐Hbs1 surveillance complex provides another level of defense against the expression of TE s in the germline of Drosophila and possibly all metazoa.