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Nuclear lamins are not required for lamina‐associated domain organization in mouse embryonic stem cells
Author(s) -
Amendola Mario,
Steensel Bas
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201439789
Subject(s) - lamin , nuclear lamina , chromatin , biology , microbiology and biotechnology , embryonic stem cell , inner membrane , stem cell , lamina , emerin , genetics , nuclear protein , gene , nucleus , anatomy , transcription factor , mitochondrion
In mammals, the nuclear lamina interacts with hundreds of large genomic regions, termed lamina‐associated domains ( LAD s) that are generally in a transcriptionally repressed state. Lamins form the major structural component of the lamina and have been reported to bind DNA and chromatin. Here, we systematically evaluate whether lamins are necessary for the LAD organization in murine embryonic stem cells. Surprisingly, removal of essentially all lamins does not have any detectable effect on the genome‐wide interaction pattern of chromatin with emerin, a marker of the inner nuclear membrane. This suggests that other components of the lamina mediate these interactions.