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Single muscle fiber proteomics reveals unexpected mitochondrial specialization
Author(s) -
Murgia Marta,
Nagaraj Nagarjuna,
Deshmukh Atul S,
Zeiler Marlis,
Cancellara Pasqua,
Moretti Irene,
Reggiani Carlo,
Schiaffino Stefano,
Mann Matthias
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201439757
Subject(s) - proteome , proteomics , biology , microbiology and biotechnology , fiber , computational biology , mitochondrion , fiber type , skeletal muscle , bioinformatics , biochemistry , chemistry , gene , anatomy , organic chemistry
Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high‐sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype‐specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type‐resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.