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IP 3 signalling regulates exogenous RNA i in C aenorhabditis elegans
Author(s) -
Nagy Anikó I,
VázquezManrique Rafael P,
Lopez Marie,
Christov Christo P,
Sequedo María Dolores,
Herzog Mareike,
Herlihy Anna E,
Bodak Maxime,
Gatsi Roxani,
Baylis Howard A
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201439585
Subject(s) - signalling , microbiology and biotechnology , chemistry , biology
RNA interference ( RNA i) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5‐trisphosphate ( IP 3 ) signalling alters RNA i sensitivity in C aenorhabditis elegans . Reducing IP 3 signalling enhances sensitivity to RNA i in a broad range of genes and tissues. Conversely up‐regulating IP 3 signalling decreases sensitivity. Tissue‐specific rescue experiments suggest IP 3 functions in the intestine. We also exploit IP 3 signalling mutants to further enhance the sensitivity of RNA i hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNA i responses, implying that RNA i responses may be influenced by an animal's physiology or environment.