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Conserved abundance and topological features in chromatin‐remodeling protein interaction networks
Author(s) -
Sardiu Mihaela E,
Gilmore Joshua M,
Groppe Brad D,
Herman Damir,
Ramisetty Sreenivasa R,
Cai Yong,
Jin Jingji,
Conaway Ronald C,
Conaway Joan W,
Florens Laurence,
Washburn Michael P
Publication year - 2015
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201439403
Subject(s) - chromatin remodeling , chromatin , biology , computational biology , abundance (ecology) , evolutionary biology , microbiology and biotechnology , topology (electrical circuits) , genetics , gene , ecology , mathematics , combinatorics
Abstract The study of conserved protein interaction networks seeks to better understand the evolution and regulation of protein interactions. Here, we present a quantitative proteomic analysis of 18 orthologous baits from three distinct chromatin‐remodeling complexes in Saccharomyces cerevisiae and Homo sapiens . We demonstrate that abundance levels of orthologous proteins correlate strongly between the two organisms and both networks have highly similar topologies. We therefore used the protein abundances in one species to cross‐predict missing protein abundance levels in the other species. Lastly, we identified a novel conserved low‐abundance subnetwork further demonstrating the value of quantitative analysis of networks.