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Tel1 ATM dictates the replication timing of short yeast telomeres
Author(s) -
Cooley Carol,
Davé Anoushka,
Garg Mansi,
Bianchi Alessandro
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201439242
Subject(s) - telomere , telomerase , biology , dna replication , microbiology and biotechnology , genetics , replication timing , origin recognition complex , control of chromosome duplication , dna , eukaryotic dna replication , gene
Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double‐strand breaks flanked by short telomeric arrays cause origin firing early in S phase at late‐replicating loci and that this effect on origin firing time is dependent on the Tel1 ATM checkpoint kinase. The effect of Tel1 ATM on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss. These results establish that Tel1 ATM specifies not only the extent but also the timing of telomerase recruitment.