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Taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured primary brown and brite/beige adipocytes
Author(s) -
Li Yongguo,
Fromme Tobias,
Schweizer Sabine,
Schöttl Theresa,
Klingenspor Martin
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.201438775
Subject(s) - thermogenesis , lipolysis , adipose triglyceride lipase , thermogenin , adipose tissue , brown adipose tissue , biology , endocrinology , white adipose tissue , medicine , adipocyte , intracellular , uncoupling protein , fatty acid , hormone sensitive lipase , beta oxidation , microbiology and biotechnology , biochemistry
Thermogenesis in brown adipocytes, conferred by mitochondrial uncoupling protein 1 ( UCP 1), is receiving great attention because metabolically active brown adipose tissue may protect humans from metabolic diseases. In particular, the thermogenic function of brown‐like adipocytes in white adipose tissue, known as brite (or beige) adipocytes, is currently of prime interest. A valid procedure to quantify the specific contribution of UCP 1 to thermogenesis is thus of vital importance. Adrenergic stimulation of lipolysis is a common way to activate UCP 1. We here report, however, that in this frequently applied setup, taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured brown and brite adipocytes. By the application of these findings, we demonstrate that UCP 1 is functionally thermogenic in intact brite adipocytes and adrenergic UCP 1 activation is largely dependent on adipose triglyceride lipase ( ATGL ) rather than hormone sensitive lipase ( HSL ).