Premium
Interactomes of SARS‐CoV‐2 and human coronaviruses reveal host factors potentially affecting pathogenesis
Author(s) -
Chen Zhen,
Wang Chao,
Feng Xu,
Nie Litong,
Tang Mengfan,
Zhang Huimin,
Xiong Yun,
Swisher Samuel K,
Srivastava Mrinal,
Chen Junjie
Publication year - 2021
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2021107776
Subject(s) - biology , covid-19 , virology , coronavirus , betacoronavirus , host (biology) , pathogenesis , pandemic , sars virus , viral pathogenesis , coronavirus infections , computational biology , genetics , virus , immunology , viral replication , disease , infectious disease (medical specialty) , outbreak , medicine , pathology
Host–virus protein–protein interactions play key roles in the life cycle of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). We conducted a comprehensive interactome study between the virus and host cells using tandem affinity purification and proximity‐labeling strategies and identified 437 human proteins as the high‐confidence interacting proteins. Further characterization of these interactions and comparison to other large‐scale study of cellular responses to SARS‐CoV‐2 infection elucidated how distinct SARS‐CoV‐2 viral proteins participate in its life cycle. With these data mining, we discovered potential drug targets for the treatment of COVID‐19. The interactomes of two key SARS‐CoV‐2‐encoded viral proteins, NSP1 and N, were compared with the interactomes of their counterparts in other human coronaviruses. These comparisons not only revealed common host pathways these viruses manipulate for their survival, but also showed divergent protein–protein interactions that may explain differences in disease pathology. This comprehensive interactome of SARS‐CoV‐2 provides valuable resources for the understanding and treating of this disease.