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RNA localization and co‐translational interactions control RAB 13 GTP ase function and cell migration
Author(s) -
Moissoglu Konstadinos,
Stueland Michael,
Gasparski Alexander N,
Wang Tianhong,
Jenkins Lisa M,
Hastings Michelle L,
Mili Stavroula
Publication year - 2020
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2020104958
Subject(s) - biology , rab , microbiology and biotechnology , gtp' , function (biology) , rna , biochemistry , gtpase , gene , enzyme
Numerous RNA s exhibit specific distribution patterns in mammalian cells. However, the functional and mechanistic consequences are relatively unknown. Here, we investigate the functional role of RNA localization at cellular protrusions of migrating mesenchymal cells, using as a model the RAB 13 RNA , which encodes a GTP ase important for vesicle‐mediated membrane trafficking. While RAB 13 RNA is enriched at peripheral protrusions, the expressed protein is concentrated perinuclearly. By specifically preventing RAB 13 RNA localization, we show that peripheral RAB 13 translation is not important for the overall distribution of the RAB 13 protein or its ability to associate with membranes, but is required for full activation of the GTP ase and for efficient cell migration. RAB 13 translation leads to a co‐translational association of nascent RAB 13 with the exchange factor RABIF . Our results indicate that RAB 13‐ RABIF association at the periphery is required for directing RAB 13 GTP ase activity to promote cell migration. Thus, translation of RAB 13 in specific subcellular environments imparts the protein with distinct properties and highlights a means of controlling protein function through local RNA translation.