Premium
Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells
Author(s) -
Rodriguez Eleazar,
Chevalier Jonathan,
Olsen Jakob,
Ansbøl Jeppe,
Kapousidou Vaitsa,
Zuo Zhangli,
Svenning Steingrim,
Loefke Christian,
Koemeda Stefanie,
Drozdowskyj Pedro Serrano,
Jez Jakub,
Durnberger Gerhard,
Kuenzl Fabian,
Schutzbier Michael,
Mechtler Karl,
Ebstrup Elise Nagel,
Lolle Signe,
Dagdas Yasin,
Petersen Morten
Publication year - 2020
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2019103315
Subject(s) - biology , reprogramming , autophagy , somatic cell , microbiology and biotechnology , genetics , cell , gene , apoptosis
Somatic cells acclimate to changes in the environment by temporary reprogramming. Much has been learned about transcription factors that induce these cell‐state switches in both plants and animals, but how cells rapidly modulate their proteome remains elusive. Here, we show rapid induction of autophagy during temporary reprogramming in plants triggered by phytohormones, immune, and danger signals. Quantitative proteomics following sequential reprogramming revealed that autophagy is required for timely decay of previous cellular states and for tweaking the proteome to acclimate to the new conditions. Signatures of previous cellular programs thus persist in autophagy‐deficient cells, affecting cellular decision‐making. Concordantly, autophagy‐deficient cells fail to acclimatize to dynamic climate changes. Similarly, they have defects in dedifferentiating into pluripotent stem cells, and redifferentiation during organogenesis. These observations indicate that autophagy mediates cell‐state switches that underlie somatic cell reprogramming in plants and possibly other organisms, and thereby promotes phenotypic plasticity.