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Bub1—the zombie protein that CRISPR cannot kill
Author(s) -
Meraldi Patrick
Publication year - 2019
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2019101912
Subject(s) - biology , zombie , crispr , microbiology and biotechnology , computational biology , genetics , gene , world wide web , computer science
The conserved Bub1 kinase was proposed to be non‐essential for the mitotic spindle assembly checkpoint based on recent findings that knocking‐out Bub1 by CRISPR /Cas9 did not impair checkpoint function in human cells. New studies now demonstrate that CRISPR /Cas9 Bub1 knockout cells still express low levels of Bub1 protein and that only removal of this remaining Bub1 pool by RNA interference substantially weakens spindle assembly checkpoint signaling.