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Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast
Author(s) -
Ruschhaupt Moritz,
Mergner Julia,
Mucha Stefanie,
Papacek Michael,
Doch Isabel,
Tischer Stefanie V,
Hemmler Daniel,
Chiasson David,
Edel Kai H,
Kudla Jörg,
SchmittKopplin Philippe,
Kuster Bernhard,
Grill Erwin
Publication year - 2019
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2019101859
Subject(s) - library science , german , biological sciences , biology , computational biology , computer science , history , archaeology
The phytohormone abscisic acid ( ABA ) regulates plant responses to abiotic stress, such as drought and high osmotic conditions. The multitude of functionally redundant components involved in ABA signaling poses a major challenge for elucidating individual contributions to the response selectivity and sensitivity of the pathway. Here, we reconstructed single ABA signaling pathways in yeast for combinatorial analysis of ABA receptors and coreceptors, downstream‐acting Sn RK 2 protein kinases, and transcription factors. The analysis shows that some ABA receptors stimulate the pathway even in the absence of ABA and that Sn RK 2s are major determinants of ABA responsiveness by differing in the ligand‐dependent control. Five Sn RK 2s, including Sn RK 2.4 known to be active under osmotic stress in plants, activated ABA ‐responsive transcription factors and were regulated by ABA receptor complexes in yeast. In the plant tissue, Sn RK 2.4 and ABA receptors competed for coreceptor interaction in an ABA ‐dependent manner consistent with a tight integration of Sn RK 2.4 into the ABA signaling pathway. The study establishes the suitability of the yeast system for the dissection of core signaling cascades and opens up future avenues of research on ligand‐receptor regulation.