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Actin filaments regulate microtubule growth at the centrosome
Author(s) -
Inoue Daisuke,
Obino Dorian,
Pineau Judith,
Farina Francesca,
Gaillard Jérémie,
Guerin Christophe,
Blanchoin Laurent,
LenDuménil AnaMaria,
Théry Manuel
Publication year - 2019
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201899630
Subject(s) - centrosome , microbiology and biotechnology , biology , microtubule , actin remodeling , actin , cytoskeleton , actin remodeling of neurons , crosstalk , actin cytoskeleton , cell , cell cycle , biochemistry , physics , optics
The centrosome is the main microtubule‐organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here, we show that increasing densities of actin filaments at the centrosome of lymphocytes are correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in disassembly of centrosomal actin and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed in vitro reconstitution assays based on (i) purified centrosomes and (ii) on the co‐micropatterning of microtubule seeds and actin filaments. These two assays demonstrated that actin filaments constitute a physical barrier blocking elongation of nascent microtubules. Finally, we showed that cell adhesion and cell spreading lead to lower densities of centrosomal actin, thus resulting in higher microtubule growth. We therefore propose a novel mechanism, by which the number of centrosomal microtubules is regulated by cell adhesion and actin‐network architecture.