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Interplay between the bacterial protein deacetylase CobB and the second messenger c‐di‐ GMP
Author(s) -
Xu Zhaowei,
Zhang Hainan,
Zhang Xingrun,
Jiang Hewei,
Liu Chengxi,
Wu Fanlin,
Qian Lili,
Hao Bingbing,
Czajkowsky Daniel M,
Guo Shujuan,
Xu Zhijing,
Bi Lijun,
Wang Shihua,
Li Haitao,
Tan Minjia,
Yan Wei,
Feng Lei,
Hou Jingli,
Tao Shengce
Publication year - 2019
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2018100948
Subject(s) - biology , cobb , second messenger system , bacterial protein , messenger rna , genetics , microbiology and biotechnology , bacteria , signal transduction , gene
As a ubiquitous bacterial secondary messenger, c‐di‐ GMP plays key regulatory roles in processes such as bacterial motility and transcription regulation. CobB is the Sir2 family protein deacetylase that controls energy metabolism, chemotaxis, and DNA supercoiling in many bacteria. Using an Escherichia coli proteome microarray, we found that c‐di‐ GMP strongly binds to CobB. Further, protein deacetylation assays showed that c‐di‐ GMP inhibits the activity of CobB and thereby modulates the biogenesis of acetyl‐CoA. Interestingly, we also found that one of the key enzymes directly involved in c‐di‐ GMP production, DgcZ, is a substrate of CobB. Deacetylation of DgcZ by CobB enhances its activity and thus the production of c‐di‐ GMP . Our work establishes a novel negative feedback loop linking c‐di‐ GMP biogenesis and CobB‐mediated protein deacetylation.