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A specialised SKI complex assists the cytoplasmic RNA exosome in the absence of direct association with ribosomes
Author(s) -
Zhang Elodie,
Khanna Varun,
Dacheux Estelle,
Namane Abdelkader,
Doyen Antonia,
Gomard Maïté,
Turcotte Bernard,
Jacquier Alain,
FromontRacine Micheline
Publication year - 2019
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.2018100640
Subject(s) - ribosome , cytoplasm , rna , messenger rna , exosome complex , translation (biology) , microbiology and biotechnology , exosome , untranslated region , biology , chemistry , microrna , biochemistry , non coding rna , gene , microvesicles
Abstract The Ski2‐Ski3‐Ski8 ( SKI ) complex assists the RNA exosome during the 3′ to 5′ degradation of cytoplasmic transcripts. Previous reports showed that the SKI complex is involved in the 3′ to 5′ degradation of mRNA s, including 3′ untranslated regions ( UTR s) and devoid of ribosomes. Paradoxically, we recently showed that the SKI complex directly interacts with ribosomes during the co‐translational mRNA decay and that this interaction is necessary for its RNA degradation promoting activity. Here, we characterised a new SKI ‐associated factor, Ska1, that associates with a subpopulation of the SKI complex. We showed that Ska1 is specifically involved in the degradation of long 3′ UTR ‐containing mRNA s, poorly translated mRNA s as well as other RNA regions not associated with ribosomes, such as cytoplasmic lnc RNA s. We further show that the overexpression of SKA 1 antagonises the SKI‐ribosome association. We propose that the Ska1‐ SKI complex assists the cytoplasmic exosome in the absence of direct association of the SKI complex with ribosomes.