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OST 1‐mediated BTF 3L phosphorylation positively regulates CBF s during plant cold responses
Author(s) -
Ding Yanglin,
Jia Yuxin,
Shi Yiting,
Zhang Xiaoyan,
Song Chunpeng,
Gong Zhizhong,
Yang Shuhua
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201798228
Subject(s) - phosphorylation , biology , microbiology and biotechnology
Abstract Cold stress is a major environmental factor that negatively affects plant growth and survival. OST 1 has been identified as a key protein kinase in plant response to cold stress; however, little is known about the underlying molecular mechanism. In this study, we identified BTF 3 and BTF 3L ( BTF 3‐like), β‐subunits of a nascent polypeptide‐associated complex ( NAC ), as OST 1 substrates that positively regulate freezing tolerance. OST 1 phosphorylates BTF 3 and BTF 3L in vitro and in vivo , and facilitates their interaction with C‐repeat‐binding factors ( CBF s) to promote CBF stability under cold stress. The phosphorylation of BTF 3L at the Ser50 residue by OST 1 is required for its function in regulating freezing tolerance. In addition, BTF 3 and BTF 3L proteins positively regulate the expression of CBF genes. These findings unravel a molecular mechanism by which OST 1‐ BTF 3‐ CBF module regulates plant response to cold stress.