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Diminished nuclear RNA decay upon Salmonella infection upregulates antibacterial noncoding RNA s
Author(s) -
Imamura Katsutoshi,
Takaya Akiko,
Ishida Yoichi,
Fukuoka Yayoi,
Taya Toshiki,
Nakaki Ryo,
Kakeda Miho,
Imamachi Naoto,
Sato Aiko,
Yamada Toshimichi,
OnoguchiMizutani Rena,
Akizuki Gen,
Tanu Tanzina,
Tao Kazuyuki,
Miyao Sotaro,
Suzuki Yutaka,
Nagahama Masami,
Yamamoto Tomoko,
Jensen Torben Heick,
Akimitsu Nobuyoshi
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201797723
Subject(s) - biology , rna , salmonella , rna binding protein , microbiology and biotechnology , bacteria , non coding rna , long non coding rna , biochemistry , genetics , gene
Cytoplasmic mRNA degradation controls gene expression to help eliminate pathogens during infection. However, it has remained unclear whether such regulation also extends to nuclear RNA decay. Here, we show that 145 unstable nuclear RNA s, including enhancer RNA s ( eRNA s) and long noncoding RNA s (lnc RNA s) such as NEAT 1v2, are stabilized upon Salmonella infection in HeLa cells. In uninfected cells, the RNA exosome, aided by the Nuclear EX osome Targeting ( NEXT ) complex, degrades these labile transcripts. Upon infection, the levels of the exosome/ NEXT components, RRP 6 and MTR 4, dramatically decrease, resulting in transcript stabilization. Depletion of lnc RNA s, NEAT 1v2, or eRNA 07573 in HeLa cells triggers increased susceptibility to Salmonella infection concomitant with the deregulated expression of a distinct class of immunity‐related genes, indicating that the accumulation of unstable nuclear RNA s contributes to antibacterial defense. Our results highlight a fundamental role for regulated degradation of nuclear RNA in the response to pathogenic infection.