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Phospholipid‐flipping activity of P4‐ ATP ase drives membrane curvature
Author(s) -
Takada Naoto,
Naito Tomoki,
Inoue Takanari,
Nakayama Kazuhisa,
Takatsu Hiroyuki,
Shin HyeWon
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201797705
Subject(s) - biology , phospholipid , membrane curvature , membrane , biochemistry , biophysics , membrane protein , microbiology and biotechnology , lipid bilayer
P4‐ ATP ases are phospholipid flippases that translocate phospholipids from the exoplasmic/luminal to the cytoplasmic leaflet of biological membranes. All P4‐ ATP ases in yeast and some in other organisms are required for membrane trafficking; therefore, changes in the transbilayer lipid composition induced by flippases are thought to be crucial for membrane deformation. However, it is poorly understood whether the phospholipid‐flipping activity of P4‐ ATP ases can promote membrane deformation. In this study, we assessed membrane deformation induced by flippase activity via monitoring the extent of membrane tubulation using a system that allows inducible recruitment of Bin/amphiphysin/Rvs ( BAR ) domains to the plasma membrane ( PM ). Enhanced phosphatidylcholine‐flippase activity at the PM due to expression of ATP 10A, a member of the P4‐ ATP ase family, promoted membrane tubulation upon recruitment of BAR domains to the PM . This is the important evidence that changes in the transbilayer lipid composition induced by P4‐ ATP ases can deform biological membranes.