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High‐resolution transcription maps reveal the widespread impact of roadblock termination in yeast
Author(s) -
Candelli Tito,
Challal Drice,
Briand JeanBaptiste,
Boulay Jocelyne,
Porrua Odil,
Colin Jessie,
Libri Domenico
Publication year - 2018
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.15252/embj.201797490
Subject(s) - biology , transcription (linguistics) , genetics , termination factor , transcription factor , general transcription factor , microbiology and biotechnology , transcription factories , rna polymerase ii , transcription factor ii f , gene , rna polymerase , rna , transcriptional regulation , promoter , gene expression , philosophy , linguistics
Transcription termination delimits transcription units but also plays important roles in limiting pervasive transcription. We have previously shown that transcription termination occurs when elongating RNA polymerase II (RNAPII) collides with the DNA‐bound general transcription factor Reb1. We demonstrate here that many different DNA‐binding proteins can induce termination by a similar roadblock (RB) mechanism. We generated high‐resolution transcription maps by the direct detection of RNAPII upon nuclear depletion of two essential RB factors or when the canonical termination pathways for coding and non‐coding RNAs are defective. We show that RB termination occurs genomewide and functions independently of (and redundantly with) the main transcription termination pathways. We provide evidence that transcriptional readthrough at canonical terminators is a significant source of pervasive transcription, which is controlled to a large extent by RB termination. Finally, we demonstrate the occurrence of RB termination around centromeres and tRNA genes, which we suggest shields these regions from RNAPII to preserve their functional integrity.